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Nucleic Acids Research 2006 34(5):1564-1570; doi:10.1093/nar/gkl046
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Published online 15 March 2006

© The Author 2006. Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org

Article

Excess of charged tRNALys maintains low levels of peptidyl-tRNA hydrolase in pth(Ts) mutants at a non-permissive temperature

Serafin Vivanco-Domínguez1, Luis Rogelio Cruz-Vera2 and Gabriel Guarneros1,*

1Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del IPN Apartado Postal 14-740, 07000 México D.F., México 2Department of Biological Sciences, Stanford University Stanford, CA 94305-5020, USA

*To whom correspondence should be addressed. Tel: +52 55 5061 3338; Fax: +52 55 5061 3392; Email: gguarner{at}cinvestav.mx

Received January 9, 2006. Revised February 11, 2006. Accepted February 21, 2006.

Cellular changes have been monitored during the suppression, mediated by the overproduction of tRNALys, of thermosensitivity in Escherichia coli strain AA7852 carrying a mutation in peptidyl-tRNA hydrolase (Pth) encoded by the pth(Ts) gene. The presence in AA7852 cells of a plasmid bearing lysV gene helped to maintain low levels of the unstable Pth(Ts) protein and to preserve the viability of the mutant line at 41°C whereas plasmids bearing other tRNA genes were ineffective. At 32°C the excess of tRNALys did not alter the percentages of the free-, charged- or peptidyl-tRNALys species compared with those found in strains that did not overproduce tRNALys. At 41°C, however, despite increases in the level of peptidyl-tRNALys, the excess tRNALys helped to maintain the concentration of charged-tRNALys at a level comparable with that found in non-overproducer cells grown at a permissive temperature. In addition, the excess tRNALys at 41°C provoked a reduction in the concentrations of various peptidyl-tRNAs, which normally accumulate in pth(Ts) cells, and a proportional increase in the concentrations of the corresponding aminoacyl-tRNAs. The possible mechanism of rescue due to the overexpression of tRNALys and the causes of tRNALys starvation in pth(Ts) strains grown at non-permissive temperatures are considered.


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Peptidyl-tRNA hydrolase and its critical role in protein biosynthesis.
Microbiology, August 1, 2006; 152(Pt 8): 2191 - 2195.
[Abstract] [Full Text] [PDF]


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