Published online 31 March 2006
Article |
The expression profile of microRNAs in mouse embryos
1 Center for Cell and Gene Therapy, Takara Bio Inc. Seta 3-4-1, Otsu, Shiga 520-2193, Japan 2 DNA Function Analysis Center, Takara Bio Inc. Seta 3-4-1, Otsu, Shiga 520-2193, Japan 3 Department of Biochemistry, Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854, USA
*To whom correspondence should be addressed. Tel: +1 732 235 4115; Fax: +1 732 235 4559; Email: inouye{at}umdnj.edu
Received December 22, 2005. Revised February 4, 2006. Accepted March 7, 2006.
MicroRNAs (miRNAs), which are non-coding RNAs 18–25 nt in length, regulate a variety of biological processes, including vertebrate development. To identify new species of miRNA and to simultaneously obtain a comprehensive quantitative profile of small RNA expression in mouse embryos, we used the massively parallel signature sequencing technology that potentially identifies virtually all of the small RNAs in a sample. This approach allowed us to detect a total of 390 miRNAs, including 195 known miRNAs covering 
80% of previously registered mouse miRNAs as well as 195 new miRNAs, which are so far unknown in mouse. Some of these miRNAs showed temporal expression profiles during prenatal development (E9.5, E10.5 and E11.5). Several miRNAs were positioned in polycistron clusters, including one particular large transcription unit consisting of 16 known and 23 new miRNAs. Our results indicate existence of a significant number of new miRNAs expressed at specific stages of mammalian embryonic development and which were not detected by earlier methods.
Correspondence may also be addressed to Ikunoshin Kato. Tel: +81 77 543 7235; Fax: +81 77 543 2312; Email: katoi{at}takara-bio.co.jp
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Okamoto, J. Mineno, H. Ikeda, H. Fujiwara, M. Yasukawa, H. Shiku, and I. Kato Improved Expression and Reactivity of Transduced Tumor-Specific TCRs in Human Lymphocytes by Specific Silencing of Endogenous TCR Cancer Res., December 1, 2009; 69(23): 9003 - 9011. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Gabory, M.-A. Ripoche, A. Le Digarcher, F. Watrin, A. Ziyyat, T. Forne, H. Jammes, J. F. X. Ainscough, M. A. Surani, L. Journot, et al. H19 acts as a trans regulator of the imprinted gene network controlling growth in mice Development, October 15, 2009; 136(20): 3413 - 3421. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-S. Luo, O. Ishibashi, G. Ishikawa, T. Ishikawa, A. Katayama, T. Mishima, T. Takizawa, T. Shigihara, T. Goto, A. Izumi, et al. Human Villous Trophoblasts Express and Secrete Placenta-Specific MicroRNAs into Maternal Circulation via Exosomes Biol Reprod, October 1, 2009; 81(4): 717 - 729. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Gutierrez, J. D. Bussell, D. I. Pacurar, J. Schwambach, M. Pacurar, and C. Bellini Phenotypic Plasticity of Adventitious Rooting in Arabidopsis Is Controlled by Complex Regulation of AUXIN RESPONSE FACTOR Transcripts and MicroRNA Abundance PLANT CELL, October 1, 2009; 21(10): 3119 - 3132. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. S. Bartolomei Genomic imprinting: employing and avoiding epigenetic processes Genes & Dev., September 15, 2009; 23(18): 2124 - 2133. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Katoh, Y. Sakaguchi, K. Miyauchi, T. Suzuki, S.-i. Kashiwabara, T. Baba, and T. Suzuki Selective stabilization of mammalian microRNAs by 3' adenylation mediated by the cytoplasmic poly(A) polymerase GLD-2 Genes & Dev., February 15, 2009; 23(4): 433 - 438. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L.-J. Xue, J.-J. Zhang, and H.-W. Xue Characterization and expression profiles of miRNAs in rice seeds Nucleic Acids Res., February 1, 2009; 37(3): 916 - 930. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Toloubeydokhti, Qun Pan, Xiaoping Luo, O. Bukulmez, and N. Chegini The Expression and Ovarian Steroid Regulation of Endometrial Micro-RNAs Reproductive Sciences, December 1, 2008; 15(10): 993 - 1001. [Abstract] [PDF]
|
|
|
|
 |
|
 T. Mishima, T. Takizawa, S.-S. Luo, O. Ishibashi, Y. Kawahigashi, Y. Mizuguchi, T. Ishikawa, M. Mori, T. Kanda, T. Goto, et al. MicroRNA (miRNA) cloning analysis reveals sex differences in miRNA expression profiles between adult mouse testis and ovary Reproduction, December 1, 2008; 136(6): 811 - 822. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Yu, J. Lin, D. J. Zack, J. T. Mendell, and J. Qian Analysis of regulatory network topology reveals functionally distinct classes of microRNAs Nucleic Acids Res., November 1, 2008; 36(20): 6494 - 6503. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. R. Mercer, M. E. Dinger, J. Mariani, K. S. Kosik, M. F. Mehler, and J. S. Mattick Noncoding RNAs in Long-Term Memory Formation Neuroscientist, October 1, 2008; 14(5): 434 - 445. [Abstract] [PDF]
|
|
|
|
 |
|
 M. E. Dinger, P. P. Amaral, T. R. Mercer, K. C. Pang, S. J. Bruce, B. B. Gardiner, M. E. Askarian-Amiri, K. Ru, G. Solda, C. Simons, et al. Long noncoding RNAs in mouse embryonic stem cell pluripotency and differentiation Genome Res., September 1, 2008; 18(9): 1433 - 1445. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Yoshimizu, A. Miroglio, M.-A. Ripoche, A. Gabory, M. Vernucci, A. Riccio, S. Colnot, C. Godard, B. Terris, H. Jammes, et al. The H19 locus acts in vivo as a tumor suppressor PNAS, August 26, 2008; 105(34): 12417 - 12422. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. A. Glazov, S. McWilliam, W. C. Barris, and B. P. Dalrymple Origin, Evolution, and Biological Role of miRNA Cluster in DLK-DIO3 Genomic Region in Placental Mammals Mol. Biol. Evol., May 1, 2008; 25(5): 939 - 948. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. R. Viswanathan, G. Q. Daley, and R. I. Gregory Selective Blockade of MicroRNA Processing by Lin28 Science, April 4, 2008; 320(5872): 97 - 100. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. E Dinger, T. R Mercer, and J. S Mattick RNAs as extracellular signaling molecules J. Mol. Endocrinol., April 1, 2008; 40(4): 151 - 159. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Huppi, N. Volfovsky, T. Runfola, T. L. Jones, M. Mackiewicz, S. E. Martin, J. F. Mushinski, R. Stephens, and N. J. Caplen The Identification of MicroRNAs in a Genomically Unstable Region of Human Chromosome 8q24 Mol. Cancer Res., February 1, 2008; 6(2): 212 - 221. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Pan, X. Luo, T. Toloubeydokhti, and N. Chegini The expression profile of micro-RNA in endometrium and endometriosis and the influence of ovarian steroids on their expression Mol. Hum. Reprod., November 1, 2007; 13(11): 797 - 806. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Tang, J. Gal, X. Zhuang, W. Wang, H. Zhu, and G. Tang A simple array platform for microRNA analysis and its application in mouse tissues RNA, October 1, 2007; 13(10): 1803 - 1822. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Choi, Y. Qin, M. F. Berger, D. J. Ballow, M. L. Bulyk, and A. Rajkovic Microarray Analyses of Newborn Mouse Ovaries Lacking Nobox Biol Reprod, August 1, 2007; 77(2): 312 - 319. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Carninci Constructing the landscape of the mammalian transcriptome J. Exp. Biol., May 1, 2007; 210(9): 1497 - 1506. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Beuvink, F. A. Kolb, W. Budach, A. Garnier, J. Lange, F. Natt, U. Dengler, J. Hall, W. Filipowicz, and J. Weiler A novel microarray approach reveals new tissue-specific signatures of known and predicted mammalian microRNAs Nucleic Acids Res., April 1, 2007; 35(7): e52 - e52. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. L. Coutinho, L. K. Matukumalli, T. S. Sonstegard, C. P. Van Tassell, L. C. Gasbarre, A. V. Capuco, and T. P. L. Smith Discovery and profiling of bovine microRNAs from immune-related and embryonic tissues Physiol Genomics, March 14, 2007; 29(1): 35 - 43. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Cai and B. R. Cullen The imprinted H19 noncoding RNA is a primary microRNA precursor RNA, March 1, 2007; 13(3): 313 - 316. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Schmitter, J. Filkowski, A. Sewer, R. S. Pillai, E. J. Oakeley, M. Zavolan, P. Svoboda, and W. Filipowicz Effects of Dicer and Argonaute down-regulation on mRNA levels in human HEK293 cells Nucleic Acids Res., October 18, 2006; 34(17): 4801 - 4815. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Takada, E. Berezikov, Y. Yamashita, M. Lagos-Quintana, W. P. Kloosterman, M. Enomoto, H. Hatanaka, S.-i. Fujiwara, H. Watanabe, M. Soda, et al. Mouse microRNA profiles determined with a new and sensitive cloning method Nucleic Acids Res., October 18, 2006; 34(17): e115 - e115. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Q. Harper, P. D. Staber, C. R. Beck, S. K. Fineberg, C. Stein, D. Ochoa, and B. L. Davidson Optimization of Feline Immunodeficiency Virus Vectors for RNA Interference J. Virol., October 1, 2006; 80(19): 9371 - 9380. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Berezikov, G. van Tetering, M. Verheul, J. van de Belt, L. van Laake, J. Vos, R. Verloop, M. van de Wetering, V. Guryev, S. Takada, et al. Many novel mammalian microRNA candidates identified by extensive cloning and RAKE analysis Genome Res., October 1, 2006; 16(10): 1289 - 1298. [Abstract] [Full Text] [PDF]
|
|