Skip Navigation

Nucleic Acids Research 2006 34(9):2558-2569; doi:10.1093/nar/gkl278
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (626K) Freely available
Right arrow Screen PDF (635K) Freely available
Right arrow Supplementary Data
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (31)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Kloosterman, W. P.
Right arrow Articles by Plasterk, R. H.A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kloosterman, W. P.
Right arrow Articles by Plasterk, R. H.A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Published online 12 May 2006

© The Author 2006. Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org

Article

Cloning and expression of new microRNAs from zebrafish

Wigard P. Kloosterman, Florian A. Steiner, Eugene Berezikov, Ewart de Bruijn, Jose van de Belt, Mark Verheul, Edwin Cuppen and Ronald H.A. Plasterk*

Hubrecht Laboratory, Centre for Biomedical Genetics Uppsalalaan 8, 3584 CT Utrecht, The Netherlands

*To whom correspondence should be addressed. Tel: +31 30 2121963; Fax: +31 30 2516554; Email: plasterk{at}niob.knaw.nl

Received February 16, 2006. Revised March 21, 2006. Accepted April 4, 2006.

MicroRNAs (miRNAs) play an important role in development and regulate the expression of many animal genes by post-transcriptional gene silencing. Here we describe the cloning and expression of new miRNAs from zebrafish. By high-throughput sequencing of small-RNA cDNA libraries from 5-day-old zebrafish larvae and adult zebrafish brain we found 139 known miRNAs and 66 new miRNAs. For 65 known miRNAs and for 11 new miRNAs we also cloned the miRNA star sequence. We analyzed the temporal and spatial expression patterns for 35 new miRNAs and for 32 known miRNAs in the zebrafish by whole mount in situ hybridization and northern blotting. Overall, 23 of the 35 new miRNAs and 30 of the 32 known miRNAs could be detected. We found that most miRNAs were expressed during later stages of development. Some were expressed ubiquitously, but many of the miRNAs were expressed in a tissue-specific manner. Most newly discovered miRNAs have low expression levels and are less conserved in other vertebrate species. Our cloning and expression analysis indicates that most abundant and conserved miRNAs in zebrafish are now known.

The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
M. L. Yeung, Y. Bennasser, K. Watashi, S.-Y. Le, L. Houzet, and K.-T. Jeang
Pyrosequencing of small non-coding RNAs in HIV-1 infected cells: evidence for the processing of a viral-cellular double-stranded RNA hybrid
Nucleic Acids Res., October 1, 2009; 37(19): 6575 - 6586.
[Abstract] [Full Text] [PDF]

Home page
 Mol Biol EvolHome page
P. F. Stadler, J. J.-L. Chen, J. Hackermuller, S. Hoffmann, F. Horn, P. Khaitovich, A. K. Kretzschmar, A. Mosig, S. J. Prohaska, X. Qi, et al.
Evolution of Vault RNAs
Mol. Biol. Evol., September 1, 2009; 26(9): 1975 - 1991.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
A. S. Flynt, E. J. Thatcher, K. Burkewitz, N. Li, Y. Liu, and J. G. Patton
miR-8 microRNAs regulate the response to osmotic stress in zebrafish embryos
J. Cell Biol., April 6, 2009; 185(1): 115 - 127.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
L. Pase, J. E. Layton, W. P. Kloosterman, D. Carradice, P. M. Waterhouse, and G. J. Lieschke
miR-451 regulates zebrafish erythroid maturation in vivo via its target gata2
Blood, February 19, 2009; 113(8): 1794 - 1804.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
A. Shkumatava, A. Stark, H. Sive, and D. P. Bartel
Coherent but overlapping expression of microRNAs and their targets during vertebrate development
Genes & Dev., February 15, 2009; 23(4): 466 - 481.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
E. P. Murchison, P. Kheradpour, R. Sachidanandam, C. Smith, E. Hodges, Z. Xuan, M. Kellis, F. Grutzner, A. Stark, and G. J. Hannon
Conservation of small RNA pathways in platypus
Genome Res., June 1, 2008; 18(6): 995 - 1004.
[Abstract] [Full Text] [PDF]

Home page
 Mol Biol EvolHome page
J. Lu, Y. Fu, S. Kumar, Y. Shen, K. Zeng, A. Xu, R. Carthew, and C.-I Wu
Adaptive Evolution of Newly Emerged Micro-RNA Genes in Drosophila
Mol. Biol. Evol., May 1, 2008; 25(5): 929 - 938.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
D. L. Ouellet, I. Plante, P. Landry, C. Barat, M.-E. Janelle, L. Flamand, M. J. Tremblay, and P. Provost
Identification of functional microRNAs released through asymmetrical processing of HIV-1 TAR element
Nucleic Acids Res., April 1, 2008; 36(7): 2353 - 2365.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
A. Cohen, M. Shmoish, L. Levi, U. Cheruti, B. Levavi-Sivan, and E. Lubzens
Alterations in Micro-Ribonucleic Acid Expression Profiles Reveal a Novel Pathway for Estrogen Regulation
Endocrinology, April 1, 2008; 149(4): 1687 - 1696.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
L. C. Dore, J. D. Amigo, C. O. dos Santos, Z. Zhang, X. Gai, J. W. Tobias, D. Yu, A. M. Klein, C. Dorman, W. Wu, et al.
A GATA-1-regulated microRNA locus essential for erythropoiesis
PNAS, March 4, 2008; 105(9): 3333 - 3338.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. M. Heimberg, L. F. Sempere, V. N. Moy, P. C. J. Donoghue, and K. J. Peterson
MicroRNAs and the advent of vertebrate morphological complexity
PNAS, February 26, 2008; 105(8): 2946 - 2950.
[Abstract] [Full Text] [PDF]

Home page
 Cold Spring Harb Symp Quant BiolHome page
A.F. SCHIER and A.J. GIRALDEZ
MicroRNA Function and Mechanism: Insights from Zebra Fish
Cold Spring Harb Symp Quant Biol, January 1, 2006; 71(0): 195 - 203.
[Abstract] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.