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Nucleic Acids Research 2006 34(9):e65; doi:10.1093/nar/gkl173
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Published online 12 May 2006

© The Author 2006. Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org

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MicroRNA enrichment among short ‘ultraconserved’ sequences in insects

T. Tran1, P. Havlak2 and J. Miller1,*

1 Department of Biochemistry, Baylor College of Medicine TX, USA 2 Department of Human Genome Sequencing Center, Baylor College of Medicine TX, USA

*To whom correspondence should be addressed. Tel: +1 713 798 3542; Fax: +1 713 796 9438; Email: millerj{at}bcm.tmc.edu

Received September 28, 2005. Revised January 2, 2006. Accepted March 20, 2006.

MicroRNAs are short (~22 nt) regulatory RNA molecules that play key roles in metazoan development and have been implicated in human disease. First discovered in Caenorhabditis elegans, over 2500 microRNAs have been isolated in metazoans and plants; it has been estimated that there may be more than a thousand microRNA genes in the human genome alone. Motivated by the experimental observation of strong conservation of the microRNA let-7 among nearly all metazoans, we developed a novel methodology to characterize the class of such strongly conserved sequences: we identified a non-redundant set of all sequences 20 to 29 bases in length that are shared among three insects: fly, bee and mosquito. Among the few hundred sequences greater than 20 bases in length are close to 40% of the 78 confirmed fly microRNAs, along with other non-coding RNAs and coding sequence.


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W. Salerno, P. Havlak, and J. Miller
Scale-invariant structure of strongly conserved sequence in genomic intersections and alignments
PNAS, August 29, 2006; 103(35): 13121 - 13125.
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