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Nucleic Acids Research 2006 34(Database Issue):D731-D735; doi:10.1093/nar/gkj077
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Nucleic Acids Research, 2006, Vol. 34, Database issue D731-D735
© The Author 2006. Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions{at}oxfordjournals.org


Article

Plant MPSS databases: signature-based transcriptional resources for analyses of mRNA and small RNA

Mayumi Nakano1, Kan Nobuta1, Kalyan Vemaraju2, Shivakundan Singh Tej2, Jeremy W. Skogen2 and Blake C. Meyers1,2,*

1Department of Plant and Soil Sciences, University of Delaware Newark, DE 19714, USA 2Delaware Biotechnology Institute, University of Delaware Newark, DE 19714, USA

*To whom correspondence should be addressed. Tel: +1 302 831 3418; Fax: +1 302 831 4841; Email: meyers{at}dbi.udel.edu

Received August 15, 2005. Revised October 11, 2005. Accepted October 11, 2005.

MPSS (massively parallel signature sequencing) is a sequencing-based technology that uses a unique method to quantify gene expression level, generating millions of short sequence tags per library. We have created a series of databases for four species (Arabidopsis, rice, grape and Magnaporthe grisea, the rice blast fungus). Our MPSS databases measure the expression level of most genes under defined conditions and provide information about potentially novel transcripts (antisense transcripts, alternative splice isoforms and regulatory intergenic transcripts). A modified version of MPSS has been used to perform deep profiling of small RNAs from Arabidopsis, and we have recently adapted our database to display these data. Interpretation of the small RNA MPSS data is facilitated by the inclusion of extensive repeat data in our genome viewer. All the data and the tools introduced in this article are available at http://mpss.udel.edu.


The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors


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