Nucleic Acids Research Advance Access originally published online on August 18, 2006
Nucleic Acids Research 2007 35(1):1-10; doi:10.1093/nar/gkl483
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Nucleic Acids Research, 2007, Vol. 35, No. 1 1-10
© 2006 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Genomics |
Recharacterization of ancient DNA miscoding lesions: insights in the era of sequencing-by-synthesis
Center for Ancient Genetics, Niels Bohr Institute and Biological Institutes, The University of Copenhagen Juliane Maries vej 30, DK-2100 Copenhagen Ø, Denmark 1 Center for Comparative Genomics and Bioinformatics, Penn State University 310 Wartik Lab, University Park, PA 16802, USA 2 Bioinformatics Research Center, University of Aarhus Aarhus DK-8000, Denmark 3 Molecular Diagnostic Laboratory, Aarhus University Hospital Aarhus DK-8200, Denmark 4 McMaster Ancient DNA Center, Department of Anthropology and Pathology & Molecular Medicine, McMaster University Hamilton, Ontario, Canada L82 4L9 5 School of Forest Resources, Penn State University 323 Forest Resources Building, University Park, PA 16802, USA 6 Department of Plant Biology, University of Georgia Athens, GA 30602-7271, USA
*To whom correspondence should be addressed. Tel: +45 35320587; Fax: +45 35365357; Email: mtpgilbert{at}gmail.com
Received May 25, 2006. Revised October 23, 2006. Accepted October 26, 2006.
Although ancient DNA (aDNA) miscoding lesions have been studied since the earliest days of the field, their nature remains a source of debate. A variety of conflicting hypotheses exist about which miscoding lesions constitute true aDNA damage as opposed to PCR polymerase amplification error. Furthermore, considerable disagreement and speculation exists on which specific damage events underlie observed miscoding lesions. The root of the problem is that it has previously been difficult to assemble sufficient data to test the hypotheses, and near-impossible to accurately determine the specific strand of origin of observed damage events. With the advent of emulsion-based clonal amplification (emPCR) and the sequencing-by-synthesis technology this has changed. In this paper we demonstrate how data produced on the Roche GS20 genome sequencer can determine miscoding lesion strands of origin, and subsequently be interpreted to enable characterization of the aDNA damage behind the observed phenotypes. Through comparative analyses on 390 965 bp of modern chloroplast and 131 474 bp of ancient woolly mammoth GS20 sequence data we conclusively demonstrate that in this sample at least, a permafrost preserved specimen, Type 2 (cytosine
thymine/guanineadenine) miscoding lesions represent the overwhelming majority of damage-derived miscoding lesions. Additionally, we show that an as yet unidentified guanineadenine analogue modification, not the conventionally argued cytosineuracil deamination, underpins a significant proportion of Type 2 damage. How widespread these implications are for aDNA will become apparent as future studies analyse data recovered from a wider range of substrates.
The paper has undergone extensive revision, this is the final version.
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