Nucleic Acids Research Advance Access originally published online on January 3, 2007
Nucleic Acids Research 2007 35(3):822-838; doi:10.1093/nar/gkl1081
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Nucleic Acids Research, 2007, Vol. 35, No. 3 822-838
© 2006 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Molecular Biology |
Control of the yeast telomeric senescence survival pathways of recombination by the Mec1 and Mec3 DNA damage sensors and RPA
UMR CNRS no. 5161, Ecole Normale Supérieure de Lyon IFR128 BioSciences Gerland, 69364 Lyon, France
*To whom correspondence should be addressed at Ecole Normale Supérieure de Lyon, UMR CNRS 5161 46, allée d'Italie, 69364 Lyon, France. Tel: +33 47272 8170; Fax: +33 47272 8080; Email: Michel.CHARBONNEAU{at}ens-lyon.fr
Received August 17, 2006. Revised November 16, 2006. Accepted November 17, 2006.
Saccharomyces cerevisiae telomerase-negative cells undergo homologous recombination on subtelomeric or TG1–3 telomeric sequences, thus allowing Type I or Type II post-senescence survival, respectively. Here, we find that the DNA damage sensors, Mec1, Mec3 and Rad24 control Type II recombination, while the Rad9 adaptor protein and the Rad53 and Chk1 effector kinases have no effect on survivor type selection. Therefore, the Mec1 and Mec3 checkpoint complexes control telomeric recombination independently of their roles in generating and amplifying the Mec1-Rad53-Chk1 kinase cascade. rfa1-t11 mutant cells, bearing a mutation in Replication Protein A (RPA) conferring a defect in recruiting Mec1-Ddc2, were also deficient in both types of telomeric recombination. Importantly, expression of an Rfa1-t11-Ddc2 hybrid fusion protein restored checkpoint-dependent arrest, but did not rescue defective telomeric recombination. Therefore, the Rfa1-t11-associated defect in telomeric recombination is not solely due to its failure to recruit Mec1. We have also isolated novel alleles of RFA1 that were deficient in Type I but not in Type II recombination and proficient in checkpoint control. Therefore, the checkpoint and recombination functions of RPA can be genetically separated, as can the RPA-mediated control of the two types of telomeric recombination.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  R. C. Burgess, S. Rahman, M. Lisby, R. Rothstein, and X. Zhao The Slx5-Slx8 Complex Affects Sumoylation of DNA Repair Proteins and Negatively Regulates Recombination Mol. Cell. Biol., September 1, 2007; 27(17): 6153 - 6162. [Abstract] [Full Text] [PDF]
|
|