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Nucleic Acids Research Advance Access originally published online on January 30, 2007
Nucleic Acids Research 2007 35(5):1391-1401; doi:10.1093/nar/gkl1144
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Nucleic Acids Research, 2007, Vol. 35, No. 5 1391-1401
© 2007 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Molecular Biology

Proteomic analysis of the U1 snRNP of Schizosaccharomyces pombe reveals three essential organism-specific proteins

Alain N. S. Newo1, Martin Lützelberger1, Claudia A. Bottner1, Jürgen Wehland2, Josef Wissing2, Lothar Jänsch2 and Norbert F. Käufer1,*

1Institute of Genetics, Technical University of Braunschweig, Spielmannstr. 7, 38106 Braunschweig, Germany and 2Helmholtz Center for Infection Research, Inhoffenstr. 7, 38124 Braunschweig, Germany

*To whom correspondence should be addressed. Tel: +49 531 391 5774; Fax: +49 531 391 5765; E-mail: n.kaeufer{at}tu-bs.de

Received May 21, 2006. Revised December 15, 2006. Accepted December 15, 2006.

Characterization of spliceosomal complexes in the fission yeast Schizosaccharomyces pombe revealed particles sedimenting in the range of 30–60S, exclusively containing U1 snRNA. Here, we report the tandem affinity purification (TAP) of U1-specific protein complexes. The components of the complexes were identified using (LC-MS/MS) mass spectrometry. The fission yeast U1 snRNP contains 16 proteins, including the 7 Sm snRNP core proteins. In both fission and budding yeast, the U1 snRNP contains 9 and 10 U1 specific proteins, respectively, whereas the U1 particle found in mammalian cells contains only 3. Among the U1-specific proteins in S. pombe, three are homolog to the mammalian and six to the budding yeast Saccharomyces cerevisiae U1-specific proteins, whereas three, called U1H, U1J and U1L, are proteins specific to S. pombe. Furthermore, we demonstrate that the homolog of U1-70K and the three proteins specific to S. pombe are essential for growth. We will discuss the differences between the U1 snRNPs with respect to the organism-specific proteins found in the two yeasts and the resulting effect it has on pre-mRNA splicing.


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