Skip Navigation


Nucleic Acids Research Advance Access originally published online on April 10, 2007
Nucleic Acids Research 2007 35(8):2661-2670; doi:10.1093/nar/gkm140
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (2232K) Freely available
Right arrow Screen PDF (347K) Freely available
Right arrow Supplementary Material
Right arrowOA All Versions of this Article:
35/8/2661    most recent
gkm140v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (1)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Brasset, E.
Right arrow Articles by Vaury, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Brasset, E.
Right arrow Articles by Vaury, C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2007, Vol. 35, No. 8 2661-2670
© 2007 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Molecular Biology

Idefix insulator activity can be modulated by nearby regulatory elements

E. Brasset1, F. Bantignies2, F. Court1, S. Cheresiz3, C. Conte4 and C. Vaury1,*

1INSERM, U384, Faculté de Médecine, BP38, 63001 Clermont-Ferrand, France, 2Institut de Génétique Humaine, UPR 1142 – CNRS, 34396 Montpellier, France, 3Institute of Cytology and Genetics, Novosibirsk, Russia and 4INSERM, U589, 31432 Toulouse, France

*To whom correspondence should be addressed. Tel: 33 4 73 17 81 71; Fax: 33 4 73 27 61 32; Email: Chantal.VAURY{at}inserm.u-clermont1.fr

Received December 1, 2006. Revised February 22, 2007. Accepted February 22, 2007.

Insulators play important roles in controlling gene activity and maintaining regulatory independence between neighbouring genes. In this article, we show that the enhancer-blocking activity of the insulator present within the LTR retrotransposon Idefix can be abolished if two copies of the region containing the insulator—specifically, the long terminal repeat (LTR)—are fused to the retrotransposon's 5' untranslated region (5' UTR). The presence of this combination of two [LTR-5' UTR] modules is a prerequisite for the loss of enhancer-blocking activity. We further show that the 5' UTR causes flanking genomic sequences to be displaced to the nuclear periphery, which is not observed when two insulators are present by themselves. This study thus provides a functional link between insulators and independent genomic modules, which may cooperate to allow the specific regulation of defined genomic loci via nuclear repositioning. It further illustrates the complexity of genomic regulation within a chromatic environment with multiple functional elements.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
D. Chetverina, E. Savitskaya, O. Maksimenko, L. Melnikova, O. Zaytseva, A. Parshikov, A. V. Galkin, and P. Georgiev
Red flag on the white reporter: a versatile insulator abuts the white gene in Drosophila and is omnipresent in mini-white constructs
Nucleic Acids Res., February 11, 2008; 36(3): 929 - 937.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.