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Nucleic Acids Research Advance Access originally published online on December 1, 2006
Nucleic Acids Research 2007 35(Database issue):D650-D653; doi:10.1093/nar/gkl954
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Nucleic Acids Research, 2007, Vol. 35, Database issue D650-D653
© 2006 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.


Articles

PEDE (Pig EST Data Explorer) has been expanded into Pig Expression Data Explorer, including 10 147 porcine full-length cDNA sequences

Hirohide Uenishi1,3,*, Tomoko Eguchi-Ogawa1,3, Hiroki Shinkai2,3, Naohiko Okumura2,3, Kohei Suzuki2,3, Daisuke Toki2,3, Noriyuki Hamasima1,3 and Takashi Awata1,3

1 Animal Genome Research Unit, Division of Animal Sciences, National Institute of Agrobiological Sciences 2 Ikenodai, Tsukuba, Ibaraki 305-8602, Japan 2 Second Research Division, STAFF-Institute 446-1 Ippaizuka, Kamiyokoba, Tsukuba, Ibaraki 305-0854, Japan 3 Animal Genome Research Program 446-1 Ippaizuka, Kamiyokoba, Tsukuba, Ibaraki 305-0854, Japan

*To whom correspondence should be addressed. Tel: +81 29 838 8627; Fax: +81 29 838 8610; Email: huenishi{at}affrc.go.jp

Received September 15, 2006. Revised October 22, 2006. Accepted October 23, 2006.

We formerly released the porcine expressed sequence tag (EST) database Pig EST Data Explorer (PEDE; http://pede.dna.affrc.go.jp/), which comprised 68 076 high-quality ESTs obtained by using full-length-enriched cDNA libraries derived from seven tissues. We have added eight tissues and cell types to the EST analysis and have integrated 94 555 additional high-quality ESTs into the database. We also fully sequenced the inserts of 10 147 of the cDNA clones that had undergone EST analysis; the sequences and annotation of the cDNA clones were stored in the database. Further, we constructed an interface that can be used to perform various searches in the database. The PEDE database is the primary resource of expressed pig genes that are supported by full-length cDNA sequences. This resource not only enables us to pick cDNA clones of interest for a particular analysis, but it also confirms and thus contributes to the sequencing integrity of the pig genome, which is now being compiled by an international consortium (http://www.piggenome.org/). PEDE has therefore evolved into what we now call ‘Pig Expression Data Explorer’.


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