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Nucleic Acids Research Advance Access originally published online on November 28, 2006
Nucleic Acids Research 2007 35(Database issue):D680-D684; doi:10.1093/nar/gkl898
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Nucleic Acids Research, 2007, Vol. 35, Database issue D680-D684
© 2006 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Articles

PlasmID: a centralized repository for plasmid clone information and distribution

Dongmei Zuo1,2, Stephanie E. Mohr2, Yanhui Hu1, Elena Taycher1, Andreas Rolfs1, Jason Kramer2, Janice Williamson1 and Joshua LaBaer1,2,*

1 Harvard Institute of Proteomics, Harvard Medical School 320 Charles Street, Cambridge, MA 02141, USA 2 DF/HCC DNA Resource Core, Harvard Medical School 320 Charles Street, Cambridge, MA 02141, USA

*To whom correspondence should be addressed. Tel: +1 6173240816; Fax: +1 6173240824; Email: joshua_labaer{at}hms.harvard.edu

Received August 15, 2006. Accepted October 11, 2006.

The Plasmid Information Database (PlasmID; http://plasmid.hms.harvard.edu) was developed as a community-based resource portal to facilitate search and request of plasmid clones shared with the Dana-Farber/Harvard Cancer Center (DF/HCC) DNA Resource Core. PlasmID serves as a central data repository and enables researchers to search the collection online using common gene names and identifiers, keywords, vector features, author names and PubMed IDs. As of October 2006, the repository contains >46 000 plasmids in 98 different vectors, including cloned cDNA and genomic fragments from 26 different species. Moreover, the clones include plasmid vectors useful for routine and cutting-edge techniques; functionally related sets of human cDNA clones; and genome-scale gene collections for Saccharomyces cerevisiae, Pseudomonas aeruginosa, Yersinia pestis, Francisella tularensis, Bacillus anthracis and Vibrio cholerae. Information about the plasmids has been fully annotated in adherence with a high-quality standard, and clone samples are stored as glycerol stocks in a state-of-the-art automated –80°C freezer storage system. Clone replication and distribution is highly automated to minimize human error. Infor-mation about vectors and plasmid clones, including downloadable maps and sequence data, is freely available online. Researchers interested in requesting clone samples or sharing their own plasmids with the repository can visit the PlasmID website for more information.


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