Nucleic Acids Research Advance Access originally published online on May 21, 2007
Nucleic Acids Research 2007 35(Web Server issue):W633-W638; doi:10.1093/nar/gkm350
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Nucleic Acids Research, 2007, Vol. 35, No. suppl_2 W633-W638
© 2007 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
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INDELSCAN: a web server for comparative identification of species-specific and non-species-specific insertion/deletion events
1Division of Biostatistics and Bioinformatics, National Health Research Institute, Miaoli County 350, Taiwan and 2Genomics Research Center, Academia Sinica, Taipei 11529, Taiwan
*To whom correspondence should be addressed. Tel: +886 2 27898730 348; Fax: +886 2 27898757; Email: trees{at}gate.sinica.edu.tw
Received January 30, 2007. Revised April 12, 2007. Accepted April 23, 2007.
Insertion and deletion (indel) events usually have dramatic effects on genome structure and gene function. Species-specific indels have been demonstrated to be associated with species-unique traits. Currently, indel identifications mainly rely on pair-wise sequence alignments (the ‘pair-wise indels’), which suffer lack of discrimination of species specificity and insertion versus deletion. Also, there is no freely accessible web server for genome-wide identification of indels. Therefore, we develop a web server—INDELSCAN— to identify four types of indels using multiple sequence alignments that include sequences from one target, one subject and 
1 out-group species. The four types of indels identified encompass target species-specific, subject species-specific, non-species-specific and target-subject pair-wise indels. Insertions and deletions are discriminated with reference to out-group sequences. The genomic locations (5'UTR, intron, CDS, 3'UTR and intergenic region) of these indels are also provided for functional analysis. INDELSCAN provides genomic sequences and gene annotations from a wide spectrum of taxa for users to select from, including nine target species (human (Homo sapiens), mouse (Mus musculus), rat (Rattus norvegicus), dog (Canis familiaris), opossum (Monodelphis domestica), chicken (Gallus gallus), zebrafish (Danio rerio), fly (Drosophila melanogaster) and yeast (Saccharomyces cerevisiae) and >35 subject/out-group species, ranging from yeasts to mammals. The server also provides analytic figures and supports indel identification from user-uploaded alignments/annotations. INDELSCAN is freely accessible at http://indelscan.genomics.sinica.edu.tw/IndelScan/.
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