Nucleic Acids Research Advance Access originally published online on May 8, 2007
Nucleic Acids Research 2007 35(Web Server issue):W66-W70; doi:10.1093/nar/gkm305
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Nucleic Acids Research, 2007, Vol. 35, No. suppl_2 W66-W70
© 2007 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Articles |
NTMG (N-terminal Truncated Mutants Generator for cDNA): an automatic multiplex PCR assays design for generating various N-terminal truncated cDNA mutants
1Department of Health Services Management, China Medical University, Taichung, Taiwan, ROC, 2Department of Information Management, Chaoyang University of Technology, Taichung, Taiwan, ROC, 3Department of Computer Science, National Chung Hsing University, Taichung, Taiwan, ROC, 4Department of Food Science, Central Taiwan University of Science and Technology, Taichung, Taiwan, ROC and 5Department of Management Information System, National Chung Hsing University, Taichung, Taiwan, ROC
*To whom correspondence should be addressed. Tel: +886-4-22874020; Fax: +886-4-22853869; Email: lytseng{at}cs.nchu.edu.tw
Received January 31, 2007. Revised April 3, 2007. Accepted April 15, 2007.
The sequential deletion method is generally used to locate the functional domain of a protein. With this method, in order to find the various N-terminal truncated mutants, researchers have to investigate the ATG-like codons, to design various multiplex polymerase chain reaction (PCR) forward primers and to do several PCR experiments. This web server (N-terminal Truncated Mutants Generator for cDNA) will automatically generate groups of forward PCR primers and the corresponding reverse PCR primers that can be used in a single batch of a multiplex PCR experiment to extract the various N-terminal truncated mutants. This saves much time and money for those who use the sequential deletion method in their research. This server is available at http://oblab.cs.nchu.edu.tw:8080/WebSDL/.
The authors wish it to be known that the first two authors should be regarded as joint First Authors