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Nucleic Acids Research Advance Access originally published online on May 29, 2008
Nucleic Acids Research 2008 36(12):3905-3915; doi:10.1093/nar/gkn291
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Nucleic Acids Research, 2008, Vol. 36, No. 12 3905-3915
© 2008 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Molecular Biology

Transcriptional regulation of the Drosophila moira and osa genes by the DREF pathway

Kumi Nakamura1,2, Hiroyuki Ida1,2 and Masamitsu Yamaguchi1,2,*

1Department of Applied Biology and 2Insect Biomedical Research Center, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan

*To whom correspondence should be addressed. Tel: +81 75 724 7781; Fax: +81 75 724 7760; Email: myamaguc{at}kit.ac.jp

Received April 4, 2008. Revised April 27, 2008. Accepted April 28, 2008.

The DNA replication-related element binding factor (DREF) plays an important role in regulation of cell proliferation in Drosophila, binding to DRE and activating transcription of genes carrying this element in their promoter regions. Overexpression of DREF in eye imaginal discs induces a rough eye phenotype in adults, which can be suppressed by half dose reduction of the osa or moira (mor) genes encoding subunits of the BRM complex. This ATP-dependent chromatin remodeling complex is known to control gene expression and the cell cycle. In the 5' flanking regions of the osa and mor genes, DRE and DRE-like sequences exist which contribute to their promoter activities. Expression levels and promoter activities of osa and mor are decreased in DREF knockdown cells and our results in vitro and in cultured cells indicate that transcription of osa and mor is regulated by the DRE/DREF regulatory pathway. In addition, mRNA levels of other BRM complex subunits and a target gene, string/cdc25, were found to be decreased by knockdown of DREF. These results indicate that DREF is involved in regulation of the BRM complex and thereby the cell cycle.


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