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Nucleic Acids Research Advance Access originally published online on June 27, 2008
Nucleic Acids Research 2008 36(13):4295-4309; doi:10.1093/nar/gkn328
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Nucleic Acids Research, 2008, Vol. 36, No. 13 4295-4309
© 2008 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Molecular Biology

The Zur of Xanthomonas campestris functions as a repressor and an activator of putative zinc homeostasis genes via recognizing two distinct sequences within its target promoters

Dong-Liang Huang, Dong-Jie Tang, Qing Liao, Heng-Cong Li, Qi Chen, Yong-Qiang He, Jia-Xun Feng, Bo-Le Jiang, Guang-Tao Lu, Baoshan Chen and Ji-Liang Tang*

Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, The Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering, and College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning Guangxi 530004, China

*To whom correspondence should be addressed. Tel: +86 771 3239566; Fax: +86 771 3239413; Email: jltang{at}gxu.edu.cn

Received November 22, 2007. Revised April 30, 2008. Accepted May 7, 2008.

It has been long considered that zinc homeostasis in bacteria is maintained by export systems and uptake systems, which are separately controlled by their own regulators and the uptake systems are negatively regulated by Zur which binds to an about 30-bp AT-rich sequence known as Zur-box present in its target promoters to block the entry of RNA polymerase. Here, we demonstrated in vivo and in vitro that in addition to act as a repressor of putative Zn2+-uptake systems, the Zur of the bacterial phytopathogen Xanthomonas campestris pathovar campestris (Xcc) acts as an activator of a Zn2+ efflux pump. The Xcc Zur binds to a similar Zur-box with ~30-bp AT-rich sequence in the promoters of the genes encoding putative Zn2+-uptake systems but a 59-bp GC-rich sequence with a 20-bp inverted repeat overlapping the promoter's –35 to –10 sequence of the gene encoding a Zn2+-export system. Mutagenesis of the inverted repeat sequence resulted in abolishment of the in vitro binding and the in vivo and in vitro activation of the export gene's promoter by Zur. These results reveal that the Xcc Zur functions as a repressor and an activator of putative zinc homeostasis genes via recognizing two distinct sequences within its target promoters.

The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors


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K. F. Smith, L. A. Bibb, M. P. Schmitt, and D. M. Oram
Regulation and Activity of a Zinc Uptake Regulator, Zur, in Corynebacterium diphtheriae
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[Abstract] [Full Text] [PDF]


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