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Nucleic Acids Research Advance Access originally published online on July 8, 2008
Nucleic Acids Research 2008 36(14):4573-4586; doi:10.1093/nar/gkn406
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Nucleic Acids Research, 2008, Vol. 36, No. 14 4573-4586
© 2008 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

RNA

The RNA-binding protein TbDRBD3 regulates the stability of a specific subset of mRNAs in trypanosomes

Antonio M. Estévez*

Instituto de Parasitología y Biomedicina ‘López-Neyra’, CSIC Avda. del Conocimiento s/n, Armilla, 18100-Granada, Spain

*To whom correspondence should be addressed. Tel: +34 958 181 652; Fax: +34 958 181632; Email: aestevez{at}ipb.csic.es

Received May 13, 2008. Revised May 31, 2008. Accepted June 3, 2008.

In trypanosomes, the apparent lack of regulation of RNA polymerase II-dependent transcription initiation poses a challenge to understand how these eukaryotes adjust gene expression to adapt to the contrasting environments they find during their life cycles. Evidence so far indicates that mRNA turnover and translation are the major control points in which regulation is exerted in trypanosomes. However, very little is known about which proteins are involved, and how do they regulate the abundance and translation of different mRNAs in different life stages. In this work, an RNA-binding protein, TbDRBD3, has been identified by affinity chromatography, and its function addressed using RNA interference, microarray analysis and immunoprecipitation of mRNA–protein complexes. The results obtained indicate that TbDRBD3 binds to a subset of developmentally regulated mRNAs encoding membrane proteins, and that this association promotes the stabilization of the target transcripts. These observations raise the possibility that TbDRBD3-mRNA complexes act as a post-transcriptional operon, and provide a framework to interpret how trypanosomes regulate gene expression in the absence of transcriptional control.


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