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Nucleic Acids Research Advance Access originally published online on September 27, 2008
Nucleic Acids Research 2008 36(18):6021-6034; doi:10.1093/nar/gkn605
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Nucleic Acids Research, 2008, Vol. 36, No. 18 6021-6034
© 2008 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Molecular Biology

Evolutionary conservation supports ancient origin for Nudt16, a nuclear-localized, RNA-binding, RNA-decapping enzyme

Melissa J. Taylor and Brenda A. Peculis*

Department of Biochemistry, University of Missouri, Columbia, MO 65211, USA

*To whom correspondence should be addressed. Tel: +1 573 884 1424; Fax: +1 573 884 4812; Email: peculisb{at}missouri.edu

Received August 6, 2008. Revised September 4, 2008. Accepted September 5, 2008.

Nudt16p is a nuclear RNA decapping protein initially identified in Xenopus (X29) and known to exist in mammals. Here, we identified putative orthologs in 57 different organisms ranging from humans to Cnidaria (anemone/coral). In vitro analysis demonstrated the insect ortholog can bind RNA and hydrolyze the m7G cap from the 5'-end of RNAs indicating the Nudt16 gene product is functionally conserved across metazoans. This study also identified a closely related paralogous protein, known as Syndesmos, which resulted from a gene duplication that occurred in the tetrapod lineage near the amniote divergence. While vertebrate Nudt16p is a nuclear RNA decapping protein, Syndesmos is associated with the cytoplasmic membrane in tetrapods. Syndesmos is inactive for RNA decapping but retains RNA-binding activity. This structure/function analysis demonstrates evolutionary conservation of the ancient Nudt16 protein suggesting the existence and maintenance of a nuclear RNA degradation pathway in metazoans.


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