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Nucleic Acids Research Advance Access originally published online on December 10, 2007
Nucleic Acids Research 2008 36(3):e14; doi:10.1093/nar/gkm1063
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Nucleic Acids Research, 2008, Vol. 36, No. 3 e14
© 2007 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Methods Online

Quantitative telomeric overhang determination using a double-strand specific nuclease

Yong Zhao, Hirotoshi Hoshiyama, Jerry W. Shay and Woodring E. Wright*

Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, Texas, USA

*To whom correspondence should be addressed. Tel: +1 214 648 2933; Fax: +1 214 648 8694; Email: woodring.wright{at}utsouthwestern.edu

Received September 17, 2007. Revised November 6, 2007. Accepted November 8, 2007.

Telomeres terminate in 3' overhangs that function in end protection and the formation of t-loops. Determining the steps and factors involved in overhang processing is compromised by the inability to easily and accurately determine overhang size in the presence of many kilobases of double-stranded telomeric DNA. We here describe the use of a double-strand specific nuclease (DSN) that entirely digests double-stranded DNA including telomeres, leaving the overhangs intact so that they can be measured.


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[Abstract] [Full Text] [PDF]


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