Skip Navigation


Nucleic Acids Research Advance Access originally published online on January 21, 2008
Nucleic Acids Research 2008 36(3):e18; doi:10.1093/nar/gkm1043
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (1050K) Freely available
Right arrow Screen PDF (280K) Freely available
Right arrowOA All Versions of this Article:
36/3/e18    most recent
gkm1043v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Gondai, T.
Right arrow Articles by Takaku, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gondai, T.
Right arrow Articles by Takaku, H.
Related Collections
Right arrow Targeted inhibition of gene function
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2008, Vol. 36, No. 3 e18
© 2008 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Methods Online

Short-hairpin RNAs synthesized by T7 phage polymerase do not induce interferon

Takuma Gondai1, Kazuya Yamaguchi1, Naoko Miyano-Kurosaki1,2, Yuichirou Habu1,3 and Hiroshi Takaku1,2,*

1Department of Life and Environmental Science, 2High Technology Research Center, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino-shi, Chiba 275-0016 Japan and 3Japanese Foundation for AIDS Prevention, 1-3-12 Misakicho, Chiyoda, Tokyo 101-0061, Japan

*To whom correspondence should be addressed. Tel: +81 47 478 0407; Fax: +81 47 471 8764; Email: hiroshi.takaku{at}it-chiba.ac.jp

Received April 5, 2007. Revised October 31, 2007. Accepted November 1, 2007.

RNA interference (RNAi) mediated by small-interfering RNAs (siRNAs) is a highly effective gene-silencing mechanism with great potential for gene-therapeutic applications. siRNA agents also exert non-target-related biological effects and toxicities, including immune-system stimulation. Specifically, siRNA synthesized from the T7 RNA polymerase system triggers a potent induction of type-I interferon (IFN) in a variety of cells. Single-stranded RNA also stimulates innate cytokine responses in mammals. We found that pppGn (n = 2,3) associated with the 5'-end of the short-hairpin RNA (shRNA) from the T7 RNA polymerase system did not induce detectable amounts of IFN. The residual amount of guanine associated with the 5'-end and hairpin structures of the transcript was proportional to the reduction of the IFN response. Here we describe a T7 pppGn (n = 2,3) shRNA synthesis that does not induce the IFN response, and maintains the full efficacy of siRNA.

The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.