Skip Navigation


Nucleic Acids Research Advance Access originally published online on October 16, 2007
Nucleic Acids Research 2008 36(Database issue):D741-D746; doi:10.1093/nar/gkm825
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (3295K) Freely available
Right arrow Screen PDF (409K) Freely available
Right arrowOA All Versions of this Article:
36/suppl_1/D741    most recent
gkm825v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Citing Articles
Right arrowScopus Links
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Roma, G.
Right arrow Articles by Stupka, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Roma, G.
Right arrow Articles by Stupka, E.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2008, Vol. 36, Database issue D741-D746
© 2007 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This article appears in the following Nucleic Acids Research issue: Database issue [View the issue table of contents]

Articles

The UniTrap resource: tools for the biologist enabling optimized use of gene trap clones

Guglielmo Roma1, Marco Sardiello1, Gilda Cobellis1,2, Pedro Cruz1, Giampiero Lago1, Remo Sanges1,3 and Elia Stupka1,3,*

1Telethon Institute of Genetics and Medicine (TIGEM), Via P. Castellino, 111, 80131, Napoli, 2Dipartimento di Patologia Generale, Seconda Universita’ di Napoli, Via De Crecchio 7, 80100 Napoli and 3CBM S.c.r.l., c/o Area Sciente Park, Basovizza- SS14, Km 163,5 Trieste, 34012, Italy

* To whom correspondence should be addressed. Tel: +39 040 375 7718; Fax: +39 040 375 7710; Email: elia.stupka{at}cbm.fvg.it

Received August 8, 2007. Revised September 20, 2007. Accepted September 20, 2007.

We have developed a comprehensive resource devoted to biologists wanting to optimize the use of gene trap clones in their experiments. We have processed 300 602 such clones from both public and private projects to generate 28 199 ‘UniTraps’, i.e. distinct collections of unambiguous insertions at the same subgenic region of annotated genes. The UniTrap resource contains data relative to 9583 trapped genes, which represent 42.3% of the mouse gene content. Among the trapped genes, 7 728 have a counterpart in humans, and 677 are known to be involved in the pathogenesis of human diseases. The aim of this analysis is to provide the wet lab researchers with a comprehensive database and curated tools for (i) identifying and comparing the clones carrying a trap into the genes of interest, (ii) evaluating the severity of the mutation to the protein function in each independent trapping event and (iii) supplying complete information to perform PCR, RT-PCR and restriction experiments to verify the clone and identify the exact point of vector insertion. To share this unique resource with the scientific community, we have designed and implemented a web interface that is freely accessible at http://unitrap.cbm.fvg.it/.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.