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Nucleic Acids Research Advance Access originally published online on January 21, 2009
Nucleic Acids Research 2009 37(4):e28; doi:10.1093/nar/gkn1034
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Nucleic Acids Research, 2009, Vol. 37, No. 4 e28
© 2009 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Methods Online

DNA modification of live cell surface

Grigory G. Borisenko1,*, Marina A. Zaitseva1, Andrey N. Chuvilin1 and Galina E. Pozmogova1,2,*

1Research Institute of Physical-Chemical Medicine and 2Bioengineering Center, Russian Academy of Science, Moscow 119312, Russia

*To whom correspondence should be addressed. Tel: +7 499 246 4293; Fax: +7 499 246 4293; Email: grigoryb{at}yahoo.com Correspondence may also be addressed to Galina E. Pozmogova. Tel: +7 499 246 4570; Fax: +7 499 246 4293; Email: pozmge{at}gmail.com

Correspondence may also be addressed to Galina E. Pozmogova. pozmge{at}gmail.com

Received November 24, 2008. Revised November 24, 2008. Accepted December 11, 2008.

We report a novel approach for the attachment of DNA fragments to the surface of live cells. By using fluorescence microscopy and flow cytometry we demonstrated that our synthetic conjugates of fatty acid with oligonucleotides can be incorporated in plasma membrane and then hybridized with complementary sequences at the cell surface. Method permits to control amount of immobilized DNA on the cell surface. All procedures can be completed within minutes and do not alter cell viability. Using this approach we tethered floating myeloid HL-60 cells to adherent A431 epitheliocytes in a sequence specific fashion. Thus, this method allows rapid and simple DNA multicoding of the cell surface and, therefore, opens new opportunities in manipulating with cell–cell interactions.


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