Skip Navigation


Nucleic Acids Research Advance Access originally published online on January 30, 2009
Nucleic Acids Research 2009 37(5):e37; doi:10.1093/nar/gkp043
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (4461K) Freely available
Right arrow Screen PDF (295K) Freely available
Right arrow Supplementary Data
Right arrowOA All Versions of this Article:
37/5/e37    most recent
gkp043v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Butala, M.
Right arrow Articles by Lee, D. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Butala, M.
Right arrow Articles by Lee, D. J.
Related Collections
Right arrow Protein-nucleic acid interaction
Right arrow Chromatin
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2009, Vol. 37, No. 5 e37
© 2009 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Methods Online

DNA sampling: a method for probing protein binding at specific loci on bacterial chromosomes

Matej Butala1,2, Stephen J. W. Busby1,* and David J. Lee1

1School of Biosciences, University of Birmingham, Birmingham B15 2TT, UK and 2Department of Biology, Biotechnical Faculty, University of Ljubljana, Vecna pot 111, Ljubljana, Slovenia

*To whom correspondence should be addressed. Tel: +44 121 414 5439; Fax: +44 121 414 5425; Email: s.j.w.busby{at}bham.ac.uk

Received December 23, 2008. Revised January 14, 2009. Accepted January 14, 2009.

We describe a protocol, DNA sampling, for the rapid isolation of specific segments of DNA, together with bound proteins, from Escherichia coli K-12. The DNA to be sampled is generated as a discrete fragment within cells by the yeast I-SceI meganuclease, and is purified using FLAG-tagged LacI repressor and beads carrying anti-FLAG antibody. We illustrate the method by investigating the proteins bound to the colicin K gene regulatory region, either before or after induction of the colicin K gene promoter.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.