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Nucleic Acids Research Advance Access originally published online on February 10, 2009
Nucleic Acids Research 2009 37(6):1984-1990; doi:10.1093/nar/gkp033
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Nucleic Acids Research, 2009, Vol. 37, No. 6 1984-1990
© 2009 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Molecular Biology

Functional four-base A/T gap core sequence CATTAG of P53 response elements specifically bound tetrameric P53 differently than two-base A/T gap core sequence CATG bound both dimeric and tetrameric P53

Bi-He Cai1, Jang-Yi Chen1,2, Mei-Hua Lu1,2, Li-Tze Chang2, Hwang-Chi Lin3, Yu-Ming Chang2 and Chung-Faye Chao1,*

1National Defense Medical Center, Institute of Life Sciences, 2National Defense Medical Center, Department of Biology and Anatomy and 3Shin Kong Wu Ho-Su Memorial Hospital, Division of Plastic Surgery, Taipei, Taiwan, ROC

*To whom correspondence should be addressed. Tel: +886 2 87923100 ext 18183; Fax: +886 2 87923171; Email: ccf{at}ndmctsgh.edu.tw

Received November 3, 2008. Revised January 8, 2009. Accepted January 9, 2009.

The consensus sequence of p53 is repeated half sites of PuPuPuC(A/T)(A/T)GPyPyPy. GtAGCAttAGCCCAGACATGTCC is a 14-3-3{sigma} promoter p53 regulation site; the first core sequence is CAttAG, and the second is CATG. Both mutants GtAGgAttAGCCCAGACATGTCC and GtAGCAttAGCCCAGACATcTCC can be activated by p53 as a 1.5-fold half site. The original p53 regulated site on the 14-3-3{sigma} promoter is a whole site, and CATTAG is a functional core sequence. The p53-binding affinity and the activity of CATTAG were lower than for the mutant CATATG core sequence. Wild-type p53 acts as a tetramer to bind to the whole site; however, it also can bind to a half site by one of its dimers. Wild-type p53 can only bind to a half site with core sequence CATG but not to CATATG. The 1.5-fold half site or whole site with core sequence CATATG can be bound by wild-type p53. A p53 mutant, A344, forms dimeric p53; it can only bind to CATG, and not to CATATG. Therefore, tetrameric and dimeric p53 can bind to a two-base A/T gap core sequence, but only tetrameric p53 can bind to a four-base A/T gap core sequence.


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[Abstract] [Full Text] [PDF]


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