Skip Navigation


Nucleic Acids Research Advance Access originally published online on February 22, 2009
Nucleic Acids Research 2009 37(7):2238-2248; doi:10.1093/nar/gkp081
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (4258K) Freely available
Right arrow Screen PDF (890K) Freely available
Right arrow Supplementary Data
Right arrowOA All Versions of this Article:
37/7/2238    most recent
gkp081v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Krasinska, L.
Right arrow Articles by Fisher, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Krasinska, L.
Right arrow Articles by Fisher, D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2009, Vol. 37, No. 7 2238-2248
© 2009 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Genome Integrity, Repair and Replication

Replication initiation complex formation in the absence of nuclear function in Xenopus

Liliana Krasinska and Daniel Fisher*

CNRS, UMR 5535 – Institut de Génétique Moléculaire de Montpellier (IGMM), 34293 Montpellier cedex 5, France

*To whom correspondence should be addressed. Tel: +33 46761 3694; Fax: +33 46704 0231; Email: fisher{at}igmm.cnrs.fr

Received January 12, 2009. Revised January 28, 2009. Accepted January 29, 2009.

In this article, we study how intercalation-induced changes in chromatin and DNA topology affect chromosomal DNA replication using Xenopus egg extracts. Unexpectedly, intercalation by ethidium or doxorubicin prevents formation of a functional nucleus: although nucleosome formation occurs, DNA decondensation is arrested, membranous vesicles accumulate around DNA but do not fuse to form a nuclear membrane, active transport is abolished and lamins are found on chromatin, but do not assemble into a lamina. DNA replication is inhibited at the stage of initiation complex activation, as shown by molecular combing of DNA and by the absence of checkpoint activation. Replication of single-stranded DNA is not prevented. Surprisingly, in spite of the absence of nuclear function, DNA-replication proteins of pre-replication and initiation complexes are loaded onto chromatin. This is a general phenomenon as initiation complexes could also be seen without ethidium in membrane-depleted extracts which do not form nuclei. These results suggest that DNA or chromatin topology is required for generation of a functional nucleus, and activation, but not formation, of initiation complexes.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.