Skip Navigation


Nucleic Acids Research Advance Access originally published online on March 3, 2009
Nucleic Acids Research 2009 37(8):2471-2482; doi:10.1093/nar/gkp055
This Article
Right arrow Full Text Freely available
Right arrow Print PDF (11781K) Freely available
Right arrow Screen PDF (1281K) Freely available
Right arrow Supplementary Data
Right arrowOA All Versions of this Article:
37/8/2471    most recent
gkp055v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Zhang, J.-l.
Right arrow Articles by Geng, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhang, J.-l.
Right arrow Articles by Geng, F.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2009, Vol. 37, No. 8 2471-2482
© 2009 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Structural Biology

Natural isoflavones regulate the quadruplex–duplex competition in human telomeric DNA

Jin-li Zhang1, Yan Fu1, Lin Zheng1, Wei Li2,*, Hao Li3, Qian Sun2, Ying Xiao1 and Feng Geng1

1Key Laboratory of Systems Bioengineering, 2Key Laboratory for Green Chemical Technology, Ministry of Education, School of Chemical Engineering & Technology, Tianjin University, Tianjin 300072 and 3Qilu Hospital of Shandong University, Jinan 250012, Shangdong Province, China

*To whom correspondence should be addressed. Tel: +86 22 27890643; Fax: +86 22 27890643; Email: liwei{at}tju.edu.cn

Received November 22, 2008. Revised January 18, 2009. Accepted January 19, 2009.

Effects of natural isoflavones on the structural competition of human telomeric G-quadruplex d[AG3(T2AG3)3] and its related Watson–Crick duplex d[AG3(T2AG3)3-(C3TA2)3C3T] are investigated by using circular dichroism (CD), ESI-MS, fluorescence quenching measurement, CD stopped-flow kinetic experiment, UV spectroscopy and molecular modeling methods. It is intriguing to find out that isoflavones can stabilize the G-quadruplex structure but destabilize its corresponding Watson–Crick duplex and this discriminated interaction is intensified by molecular crowding environments. Kinetic experiments indicate that the dissociation rate of quadruplex (kobs290 nm) is decreased by 40.3% at the daidzin/DNA molar ratio of 1.0 in K+, whereas in Na+ the observed rate constant is reduced by about 12.0%. Furthermore, glycosidic daidzin significantly induces a structural transition of the polymorphic G-quadruplex into the antiparallel conformation in K+. This is the first report on the recognition of isoflavones with conformational polymorphism of G-quadruplex, which suggests that natural isoflavone constituents potentially exhibit distinct regulation on the structural competition of quadruplex versus duplex in human telomeric DNA.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.