Nucleic Acids Research, 2009, Vol. 37, Database issue D762-D766
© 2008 The Author(s)
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This article appears in the following Nucleic Acids Research issue: Database issue [View the issue table of contents]
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Human Gene and Protein Database (HGPD): a novel database presenting a large quantity of experiment-based results in human proteomics
1Japan Biological Informatics Consortium (JBIC), Aomi, Koto-ku, Tokyo 135-8073, 2National Institute of Advanced Industrial Science and Technology (AIST), Aomi, Koto-ku, Tokyo 135-0064, 3Reverse Proteomics Research Institute, Kisarazu, Chiba 292-0818, Japan, 4Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033, 5Life Science Research Laboratory, Central Research Laboratory, Hitachi, Ltd., Kokubunji, Tokyo 185-8601, 6Database Center for Life Science Research Organization of Information and Systems, Yayoi, Bunkyo-ku, Tokyo 113-0032 and 7Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo, Shiroganedai, Minato-ku, Tokyo 108-8639, Japan
*To whom correspondence should be addressed. Tel: +81 3 3599 8137; Fax: +81 3 3599 8141; Email: nomura.88{at}aist.go.jp
Correspondence may also be addressed to Takao Isogai, Tel: +81 3 5841 4775; Fax: +81 3 5841 4775; Email: tisogai{at}mol.f.u-tokyo.ac.jp; Naoki Goshima, Tel: +81 3 3599 8136; Fax: +81 3 3599 8141; Email: n-goshima{at}aist.go.jp
Received August 14, 2008. Revised September 29, 2008. Accepted October 17, 2008.
Completion of human genome sequencing has greatly accelerated functional genomic research. Full-length cDNA clones are essential experimental tools for functional analysis of human genes. In one of the projects of the New Energy and Industrial Technology Development Organization (NEDO) in Japan, the full-length human cDNA sequencing project (FLJ project), nucleotide sequences of approximately 30 000 human cDNA clones have been analyzed. The Gateway system is a versatile framework to construct a variety of expression clones for various experiments. We have constructed 33 275 human Gateway entry clones from full-length cDNAs, representing to our knowledge the largest collection in the world. Utilizing these clones with a highly efficient cell-free protein synthesis system based on wheat germ extract, we have systematically and comprehensively produced and analyzed human proteins in vitro. Sequence information for both amino acids and nucleotides of open reading frames of cDNAs cloned into Gateway entry clones and in vitro expression data using those clones can be retrieved from the Human Gene and Protein Database (HGPD, http://www.HGPD.jp). HGPD is a unique database that stores the information of a set of human Gateway entry clones and protein expression data and helps the user to search the Gateway entry clones.
The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors.
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