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Nucleic Acids Research, 1977, Vol. 4, No. 1 17-30
© 1977

Articles

Requirement of chain initiation factor 3 and ribosomal protein S1 in translation of synthetic and natural messenger RNA

J.E. Sobura, M. R. Chowdhury, D. A. Hawley and A.J. Wahba

Laboratory of Molecular Biology, University of Sherbrooke School of Medicine Sherbrooke, Quebec, Canada

Received August 5, 1976. Amino acid incorporation directed by poly(A), poly(U) or R17 RNA has been examined in S1-depleted protein synthesizing systems. We observe that the translation of either synthetic or natural messenger RNA is strictly dependent on the presence of chain initiation factor 3 and ribosomal protein S1. With poly(A) or poly(U) both IF-3 and S1 stimulate amino acid incorporation at least 25-fold, and with R17 RNA the stimulation is approximately 15-fold. More than one copy of S1 per ribosome decreases amino acid incorporation directed by poly(U) or R17 RNA. Initiation complex formation with R17 RNA is also stimulated optimally by the addition of one copy of S1 per ribosome. The function of IF-3 and S1 in protein synthesis is considered.


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