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Nucleic Acids Research, 1977, Vol. 4, No. 1 195-205
© 1977

Articles

5' terminal noncoding sequence heterogeneity in reovirus mRNA

Kenneth E. M. Hastings and Stewart Millward

Department of Biochemistry, McGill University Medical School Montreal, Quebec H3G 1Y6, Canada

Received October 20, 1976. The nucleotide sequences of the mRNAs of reovirus appear to diverge near the 5' termini. Ribonuclease T1 digestion of methylated mRNA synthesized in vitro yielded seven different 5' terminal fragments of the form m7G5'ppp5'GmpCpUp(Np)nGp. Chain length analysis showed that the parameter "n" in this structural formula assumes the values 3, 4 and 5.


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