Skip Navigation

This Article
Right arrow Print PDF (215K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Maxwell, I. H.
Right arrow Articles by Hahn, W. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Maxwell, I. H.
Right arrow Articles by Hahn, W. E.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1977, Vol. 4, No. 1 241-246
© 1977

Articles

Removal of RNase activity from DNase by affinity chromatography on agarose-coupled aminophenylphosphoryl-uridlne-2'(3')-phosphate

Ian H. Maxwell, Francoise Maxwell and William E. Hahn

Dempartment of Anatomy, University of Colorado School of Medicine Denver, CO 80262, USA

Received November 11, 1976. Severe degradation of high molecular weight RNA was shown to occur during incubation with commercially purified DNase. Most of the RNase activity could be removed by passage of the DNase through a column of agarose-coupled amino-phenylphosphoryl-uridine-2'(3')-phosphate. Incubation with the treated DNase caused only minimal alteration of the sedimentation pattern of high molecular weight nuclear RNA, determined under partially denaturing conditions. No impairment of DNase activity was detected.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Genes Dev.Home page
S Pinol-Roma, Y D Choi, M J Matunis, and G Dreyfuss
Immunopurification of heterogeneous nuclear ribonucleoprotein particles reveals an assortment of RNA-binding proteins.
Genes & Dev., February 1, 1988; 2(2): 215 - 227.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.