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Nucleic Acids Research, 1977, Vol. 4, No. 1 247-268
© 1977

Articles

Characterization of foldback sequences in hamster DNA using electron microscopy

Anne J. Bell and Norman Hardman

Department of Biochemistry, University of Aberdeen, Marischal College Aberdeen AB9 1AS, UK.

Received November 26, 1976. Foldback sequences in nuclear DNA from cultured Hamster fibroblasts (BHK-21/C13 cells) have been characterized by electron microscopy. One half of the structures observed when denatured hamster DNA is allowed to anneal in the range 0 < C 0t1 < 1 x 10–4 M sec result from the annealing of inverted sequences forming foldback DNA. The remainder have a probable bimolecular origin, arising from rapidly-annealing sequences of satellite-like complexity. The average length of the inverted sequences in the foldback molecules is about 0.9 kilobases. There is estimated to be about 42,000 such sequences (21,000 pairs) in the hamster genome, approximately 45% of which form looped structures with a mean loop length of 1.74 kilobases.

Contrary to previous reports, binding of the renatured duplex molecules to hydroxyapatite results in a poor recovery of structures containing identifiable foldback sequences, due to preferential enrichment of the bound fraction with duplexes formed by inter-molecular annealing


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