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Nucleic Acids Research, 1977, Vol. 4, No. 10 3341-3355
© 1977

Articles

A simple method for the preparation of (ß-32P)purine nucleoside triphosphates

Yasuhiro Furuichi and Aaron J. Shatkin

Roche Institute of Molecular Biology Nutley, NJ 07110, USA

Received July 22, 1977. A rapid, simple and inexpensive procedure is described for the preparation of purine ribo- and deoxyribonucleoside triphosphates specifically and highly radiolabeled with (32p)phosphate in the ß position. The method involves two successive enzymatic reactions: conversion of donor ({gamma}-32p)ATP in the presence of an excess of acceptor 5'-mononucleotide to the 5'-diphosphates by myokinase or guanosine 5'-monophosphate kinase followed by phosphorylation with pyruvate kinase to yield 5'-triphosphates.


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