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Nucleic Acids Research, 1977, Vol. 4, No. 10 3415-3439
© 1977

Articles

Sequence specific interaction of the chromosomal proteins with DNA

Dorothy Tuan*, Christopher Chetsanga** and Paul M. Doty

Department of Chemistry, Harvard University Cambridge, MA 02138, USA

To whom correspondence should be addressed

Received May 27, 1977.

Calf thymus chromatin was partially deproteinized by 0.6–1 M NaCl extraction. From the shape of the temperature derivative plot of its melting curve, the DNA in each chromatin was resolved into regions of exposed DNA, and DNA still complexed with proteins. The partially deproteinized chromatin was capable of directing in vitroRNA synthesis in an amount proportional to the fraction of exposed DNA. The in vitro RNA transcript was hybridized to denatured calf DNA under hybridization conditions where only RNA's transcribed from repetitive DNA sequences were able to from hybrids. From the results, it is deduced that the in vitroRNA transcripts of 0.6 M NaCl-extracted chromatin after the removal of HI histones plus one-quarter of the non histones may be transcribed from repetitive DNA sequences and those of 1 M NaCl-extracted chromatin contain more transcripts from the unique DNA sequences.

Present address Department of Biochemistry, Johns Hopkins School of Hygiene and Public Health 615 North Wolfe Street Baltimore, Maryland 21205


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