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Nucleic Acids Research, 1977, Vol. 4, No. 10 3497-3510
© 1977

Articles

Study of yeast mitochondrial tRNAs by two-dimensional polyacrylamide gel electrophoresis: characterization of isoaccepting species and search for imported cytoplasmic tRNAs

Robert P. Martin, Jean-Marie Schneller*, André J. C. Stahl* and Guy Dirheimer

Institut de Biologie Moléculaire et Cellulaire du CNRS 15 rue Descartes, 67084 Strasbourg Cedex *Laboratoire de Biochimie, Faculté de Pharmacie, Université Louis Pesteur 3 rue de 1'Argonne, 67083 Strasbourg Cedex, France

Received July 11, 1977. By two-dimensional polyacrylamide gel electrophoresis, yeast mitochondrial tRNA is fractionated into 27 major species. All but 6 of them migrate distinctly from cytoplasmic tRNAs. Migration of mitochondrial DNA-coded mitochondrial tRNAs shows the occurence of only one cytoplasmic tRNA in mitochondria. Several mitochondrial tRNA spots are identified on the electrophoregrams, some of them show isoaccepting species (Val, Ser, Met, Leu). It is suggested that there are sufficient mitochondrial tRNA genes on yeast mitochondrial DNA to allow mitochondrial protein biosynthesis by the mitochondrial tRNAs alone. Guanosine + Cytidine content and rare base composition are reported for some individual species. Mitochondrial tRNAPhe lacks ribothymidine.


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