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Nucleic Acids Research, 1977, Vol. 4, No. 10 3599-3615
© 1977

Articles

An NMR study of the exchange rates for protons involved in the secondary and tertiary structure of yeast tRNAphe

Paul D. Johnston* and Alfred G. Redfield{dagger}

*Department of Biochemistry, Brandeis University Waltham, MA 02154 {dagger}Departments of Biochemistry and Physics, and Rosenstiel Basic Medical SciencesX Research Center, Brandeis University Waltham, MA 02154, USA

Received August 5, 1977. Solvent exchange rates of all the protons of yeast tRNAphe resonating in the lowfield NMR region (–11 to –15 ppm from DSS) have been measured by saturation-recovery long-pulse Fourier transform NMR. All these protons in yeast tRNAphe are in the fast exchange limit with H2O relative to their intrinsic longitudinal relaxation processes. Most rates show very little temperature dependence; however, tertiary base pairprotons are preferentially destabilized in the absence of Mg+4 at higher temperatures. The measured exchange rates are between 2 and 125 sec–1 for a temperature range from 10°C to 45°C and MgC12 concentrations between 0 and 15 mM.


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