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Nucleic Acids Research, 1977, Vol. 4, No. 11 3743-3752
© 1977

Articles

In vitro transcription of E. coli tRNA genes

Jacob I. Grimberg and Violet Daniel

Department of Biochemistry, Weizmann Institute of Science Rehovot, Israel

Received July 20, 1977. Transcription of tRNA genes carried by transducing bacteriophages {theta}80psu3+ (Formula) and {lambda}h80T (Formula, tRNA2Glysu36+, tRNA3Thr) was studied in vitro in a system consisting of whole bacteriophage DMA and purified RNA polymerase. In contrast to unusual requirements for Formula gene transcription from DNA fragments, the transcription on whole bacteriophage DNA was found to be relatively not salt sensitive, did not require glycerol and rifampicin-resistant complexes with RNA polymerase were formed in the absence of nucleoside triphosphates. Termination factor {rho} stimulated the transcription of the tRNA genes as well as that of a 4S RNA on {lambda}h180T DNA template. The stimulatory effect of {rho} was abolished by rifampicin and seems to be due to the release of RNA polymerase and reinitiation of transcription.


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