Skip Navigation

This Article
Right arrow Print PDF (450K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Smagowicz, J.W.
Right arrow Articles by Scheit, K.H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Smagowicz, J.W.
Right arrow Articles by Scheit, K.H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1977, Vol. 4, No. 11 3863-3876
© 1977

Articles

Steady state kinetic studies of initiation of RNA synthesis on T7 DNA in the presence of rifampicin

J.W. Smagowicz and K.H. Scheit

Max-Planck-Institut für Biophysikalische Chemie Abteilung Molekulare Biologie, D-3400 Göttingen, GFR

Received August 19, 1977. The steady state kinetics of initiation of T7 DNA transcription by RNA polymerase holo enzyme from E.coli in the presence of rifampicin and the two substrates ATP and UTP were studied. Under these conditions, the enzyme catalyzes exclusively the promotor specific synthesis of pppApU. The kinetic data are in agreement with the mechanism of a truly ordered reaction. Binding of the initiating nucleotide ATP to the transcriptional complex occurs prior to the binding of the substrate UTP. Release of pppApU ist most probable the rate limiting step. Km constants were found to be 0.6 mM for ATP and 0.31 mM for UTP, respectively. The substrate inhibition pattern indicated that the substrate site exhibits a finite affinity for incorrect nucleoside triphosphate (K1 = 2.3 mM). A similar non specific binding to the 3-OH site could not be demonstrated.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.