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Nucleic Acids Research, 1977, Vol. 4, No. 11 3959-3968
© 1977

Articles

Capping structures of simian virus 40 19S and 16S mRNAs

Yoram Groner*, Pnina Carmi* and Yosef Aloni**

*Departments of Virology, The Weizmann Institute of Science Rehovot, Israel **Departments of Genetics, The Weizmann Institute of Science Rehovot, Israel

Received September 2, 1977. In vivo [methyl 3H]-labeled SV40 19S and 16S mRNA species were purified and their internal methylation as well as their capping structures analyzed. SV40 viral mRNA sedimenting in the 19S region contains approximately equal proportions of m7GpppAm and m7Gpppm6Am, while the 16S mRNA contains mainly m7Gpppm6Am. N6 methyl adenosine is located internally within the RNA chains of both the 19S and 16S species.


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