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Nucleic Acids Research, 1977, Vol. 4, No. 12 4165-4174
© 1977

Articles

End labeling of enzymatically decapped mRNA

Argiris Efstratiadis*, John N. Vournakis+, Helen Donis-Keller*, George Chaconas++, Don K. Dougall+++ and Fotis C. Kafatos*

*Harvard University Biological Laboratories Cambridge, MA 02138 +Department of Biology, Syracuse University Syracuse, NY 13210 ++Division of Medical Biochemistry, University of Calgary Alberta, Canada T2N 1N4 +++Alton Jones Cell Science Center Lake Placid, NY 12946, USA

Received October 3, 1977. A method is presented for rapid and efficient 5' end label ing with 32 of capped mRNAs, by a series of three enzymatic reactions: the blocking nucleotide of the cap structure is removed by tobacco acid pyrophosphatase, and after dephosphorylation with alkaline phosphatase the 5' end is labeled with {gamma}-32-P-ATP and T4 polynucleotide kinase.


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