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Nucleic Acids Research, 1977, Vol. 4, No. 12 4291-4304
© 1977


Articles

Nucleotide sequence of a lysine tRNA from Bacillus subtilis

Yuko Yamada and Hisayuki Ishikura*

Laboratory of Chemistry, Jichi Medical School Minamikawachi-machi, Tochigi-ken, 329-04, Japan

*To whom all correspondence should be addressed

Received September 15, 1977. A lysine tRNA (Formula) was purified from Bacillus subtilis W168 by a consequtive use of several column chromatographic systems. The nucleotide sequence was determined to be pG-A-G-C-C-A-U-U-A-G-C-U-C-A-G-U-D-G-G-D-A-G-A-G-C-A-U-C-U-G-A-C-U-U(U*)-U-U-K-A-Y-C-A-G-A-G-G-m7G(G)-U-C-G-A-A-G-G-T- Y-C-G-A-G-U-C-C-U-U-C-A-U-G-G-C-U-C-A-C-C-AOH, where K and U* are unidentified nucleosides. The nucleosides of U34 and m7G46 were partially substituted with U* and G, respectively. The binding ability of lysyl-Formula to Eecherichia coli ribosomes was stimulated with ApApA as well as ApApG.


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