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Nucleic Acids Research, 1977, Vol. 4, No. 12 4371-4390
© 1977

Articles

The nucleotide sequence at the termini of adenovirus type 5 DNA

P.H. Steenbergh, J. Maat*, H. van Ormondt* and J.S. Sussenbach

Laboratory for Physiological Chemistry, State University Utrecht, Netherlands *Laboratory for Physiological Chemistry, State University Leiden, Netherlands

Received October 27, 1977. The sequences of the first 194 base pairs at both termini of adenovirus type 5 (Ad5) DNA have been determined, using the chemical degradation technique developed by Maxam and Gilbert (Proc. Nat. Acad. Sci. USA 74 (1977), pp. 560–564).

The nucleotide sequences 1–75 were confirmed by analysis of labeled RNA transcribed from the terminal HhaI fragments in vitro. The sequence data show that Ad5 DNA has a perfect inverted terminal repetition of 103 base pairs long.


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