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Nucleic Acids Research, 1977, Vol. 4, No. 12 4391-4410
© 1977

Articles

Rapid synthesis of oligodeoxyribonucleotides II1. Machine-aided solid-phase syntheses of two nonanucleotides and an octanucleotide

M.J. Gait and R.C. Sheppard

MRC Laboratory of Molecular Biology Hills Road, Cambridge CB2 2OH, UK

Received November 1, 1977. Preparation of the two nonanucleotides, d(pG-G-A-G-G-A-G-A-A) and d(pT-T-C-T-G-T-T-G-A), and the octanucleotide, d(pT-G-G-C-C-C-A-T) is described by a rapid solid-phase method on a polyamide support. The syntheses were carried out using a modified solid-phase peptide synthesiser and required one day for each nucleotide addition. A microparticulate anion-exchanger, Partisil 10SAX, was used for the efficient fractionation of synthetic, base-protected oligonucleotides.


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