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Nucleic Acids Research, 1977, Vol. 4, No. 2 327-338
© 1977

Articles

Fluoresceinylthiocarbarnyl-tRNATyr: a useful derivative of tRNATyr (E.coli) for physico-chemical studies

Alfred Pingoud, Reinhard Kownatzki and Guenter Maass

Institut für Klinische Biochemie und Physiologische Chemie, Abteilung Biophysikalische Chemie Medizinische Hochschule, Hannover, GFR

Received November 3, 1976. Fluoresceinylthiocarbamyl-tRNATyr (FTC-tRNATyr) is prepared from tRNATyr and fluoresceinisothiocyanate (FITC) under mildly alkaline conditions. Labelling occurs specificly at the base Q of tRNATyr. The modified tRNA is fully active in the aminoacyla-tion assay; when aminoacylated it is recognized by the elongation factor Tu (EF-Tu). Codon-anticodon interaction, however, is severely affected by the modification.


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