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Nucleic Acids Research, 1977, Vol. 4, No. 2 373-380
© 1977


Articles

Phosphorylation of tRNA by T4 polynudeotide kinase

Johan R. Lillehaug and Kjell Kleppe*

Department of Biochemirtry, University of Bergen Bergen, Norway

*Author to whom correspondence should be addressed

Received November 24, 1976. The phosphorylation of various intact tRNA species by T4, po-lynucleotide kinase has been studied. The apparent Michaelis constant was on the average found to be 100 times lower than for some single-stranded DNAs previously studied. (J.R. Lille-haug and K. Kleppe, (1975) Biochemistry, 14, 1221). Conditions which result in complete phosphorylation of different tRNA species have also been established. Studies on equilibrium constants and the reversibility of the reaction revealed that the phosphorylation reaction is not a true equilibrium reaction under the conditions used in this work.


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