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Nucleic Acids Research, 1977, Vol. 4, No. 2 415-423
© 1977

Articles

Isolation of mammalian tRNAAsp and tRNATyr by lectin-Sepharose affinity column chromatography

Norihiro Okada, Nobuko Shindo-Okada and Susumu Nishimura

Biology Division, National Cancer Center Research Institute Tsukiji 5-chome Chuo-ku, Tokyo, Japan

Received December 13, 1976. tRNAAsp from rabbit liver, rat liver and rat ascites hepatoma was readily isolated by concanavalin A-Sepharose (Con A-Sepharose) affinity column chromatography. tRNATyr from these sources was extensively purified by Ricinua communis lectin-Sepharose column chromatography. These results, together with the chromatographic behaviour of four tRNAs (tRNATyr, tRNAHis, tRNAAsn and tRNAAsP) on acetylated DBAE-cellulose column chromatography suggested that tRNAAsP contains a Q* nucleoside species having a mannose moiety while tRNATyr contains Q* nucleoside with galactose. The sugars attached in 4-position of cyclopentene diol in the Q molecule are therefore not present at random in the four tRNAs, but present only in each specific tRNA. This is the first case which shows that plant agglutinin interacts with nucleic Acid as well as polysaccharide and glycoproteins.


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