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Nucleic Acids Research, 1977, Vol. 4, No. 2 461-471
© 1977

Articles

Direct evidence that ribosome bound RNA-dependent RNA polymerase does not play a role in globin messenger RNA replication

Charles D. Boyd* and W. Fitschen

Department of Biochemistry, University of Cape Town Rondebosch, Cape Town, Republic of South Africa

Received January 7, 1977.

The radioactively labelled product of RNA-dependent RNA polymerase+ from ribosomes of immature chicken erythrocytes was tested for the presence of newly replicated globin mRNA using unlabelled globin complementary DNA. No radioactively labelled globin mRNA sequences were found in the product, providing direct confirmation that this RNA-dependent RNA polymerase is not involved in globin mRNA amplification.

*Present address: Department Medical Biochemistry, University of Stellenbosch Medical School, P.O. Box 63, Tygerberg 7505, Republic of South Africa


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