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Nucleic Acids Research, 1977, Vol. 4, No. 3 503-511
© 1977

Articles

Proflavine sensitivity of RNA processing in isolated nuclei

A. Yannarell, M. Niemann, D.E. Schumm and T.E. Webb

Department of Physiological Chemistry, Ohio State University College of Medicine Columbus, OH 43210, USA

Received November 4, 1976. The intercalating agent proflavine inhibits the processing and subsequent release of preformed messenger RNA and ribosomal RNA from isolated liver nuclei to surrogate cytoplasm. The direct effect of proflavine on these processes, as monitored in a reconstituted cell-free system, supports the theory that base-paired segments (i.e. hairpin loops) in the precursor RNA's are involved as recognition sites in nuclear RNA processing.


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