Skip Navigation

This Article
Right arrow Print PDF (596K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Rosenthal, L. J.
Right arrow Articles by Brown, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rosenthal, L. J.
Right arrow Articles by Brown, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1977, Vol. 4, No. 3 551-565
© 1977

Articles

The control of SV40 transcription during a lytic infection: late RNA synthesis in the presence of inhibitors of DNA replication

Leonard J. Rosenthal* and Myles Brown

Department of Microbiology, Georgetown University, Schools of Medicine and Dentistry, Washington DC 20007 and Laboratory of DNA Tumor Viruses, National Cancer Institute Bethesda, MD20014, USA

Received November 24, 1976.

The transition from early to late transcription of SV40 DNA in productively infected BSC-1 cells was analyzed using both inhibitors of DNA replication, and early (Group A) temperature sensitive (ts) mutants of SV40. Late virus-specific cytoplasmic RNA sedimenting at 16S in neutral sucrose gradients and complementary to the plus (L) DNA strand of SV40 was detected in cultures infected in the presence of three inhibitors of DNA replication (Ara-C, FdU, and chloroquine), even though the inhibition of viral DNA replication appeared to be essentially complete. After infection with the early SV40 mutant tsA58, no DNA replication was detected at the restrictive temperature (41°C) and no significant late RNA complementary to the plus (L) strand was found, in either the cytoplasm or nuclei of infected cells. These data support the concept that expression of late viral functions requires the initiation of viral DNA synthesis or a functional gene A protein, or both.

*Author to whom reprint requests should be sent


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Virol.Home page
R. Daniels, N. M. Rusan, A.-K. Wilbuer, L. C. Norkin, P. Wadsworth, and D. N. Hebert
Simian virus 40 late proteins possess lytic properties that render them capable of permeabilizing cellular membranes.
J. Virol., July 1, 2006; 80(13): 6575 - 6587.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
M. L. Farrell and J. E. Mertz
Cell Type-Specific Replication of Simian Virus 40 Conferred by Hormone Response Elements in the Late Promoter
J. Virol., June 5, 2002; 76(13): 6762 - 6770.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.