Nucleic Acids Research, 1977, Vol. 4, No. 3 567-572
© 1977
Articles |
Lac repressor purification without inactivation of DNA binding activity
Norman W.Church Laboratory of Chemical Biology, California Institute of Technology Pasadena, CA 91125 Department of Biology, City of Hope National Medical Center Duarte, CA 91010, USA
Received November 29, 1976. A procedure has been developed which eliminates the commonly observed inactivation of the DNA binding activity of the lac repressor during purification. The operator binding activity of the repressor obtained by this method is 100 ±10%. The repressor can be stored frozen indefinitely without losing its affinity for DNA.
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