Skip Navigation

This Article
Right arrow Print PDF (301K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Kyogoku, Y.
Right arrow Articles by Nishimura, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kyogoku, Y.
Right arrow Articles by Nishimura, S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1977, Vol. 4, No. 3 585-593
© 1977

Articles

Proton magnetic resonance spectra of tRNAfMet from Thermus thermophilus

Y. Kyogoku, T. lnubushi*, I. Morishima*, K. Watanabe{dagger}, T. Oshima{dagger} and S. Nishimura+

*Department of Hydrocarbon Chemistry, Faculty of Engineering, Kyoto University Kyoto 606, Japan Institute for Protein Research, Osaka University Suita, Osaka 565

Received December 7, 1976.

220 MHz proton magnetic resonance spectra of tRNAs in bulk and tRHAfMet from Thermus therwophilus have been measured and compared with those of tRNAs from E. coli. Temperature dependences and chemical shift positions of the bulk tRNAs are well explained by the difference in their GC contents. It is known that the base sequence of the double helical regions in the cloverleaf structure of T. thermophilus tRNAfMet is different from that of E. coli tRNAfMet only at two positions in T{psi}C arm; one more C:G pair is contained instead of a U:G pair of E. coll tRNAfMet and a C:G pair of E. coli is replaced by a G:C pair. In spite of the resembrance in the base sequences, nmr patterns around 13 ppm are fairly different from each other. The difference is discussed in relation with their tertiary structures and with the origin of chemical shift displacements.

{dagger}Mitsubishi-Kasei Institute of Life SciencesMinamiooya 11, Machida-shi, Tokyo 194

+Biology Division, National Cancer Center Research InstituteTsukiji, Chuo-ku, Tokyo 4, Japan


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.