Skip Navigation

This Article
Right arrow Print PDF (560K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Slegers, H.
Right arrow Articles by Kondo, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Slegers, H.
Right arrow Articles by Kondo, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1977, Vol. 4, No. 3 625-639
© 1977

Articles

Messenger ribonucleoprotein complexes of cryptobiotic embryos of Artemia salina

Herman Slegers and Masatoshi Kondo*

Department of Cell Biology, University of Antwerp B-2610 Wilrijk, Belgium

*The author to whom all correspondence should be addressed.

Received December 20, 1976. Poly(A)-containing ribonucleoprotein [poly(A)+•RNP) particles in the post-mitochondrial supernatant of cryptobiotic embryos of Artemia salina were characterized by hybridization to [3H]poly(U). By sucrose isopycnic centrifugation, approximately 2/3 of poly(A)+•RNPs was found to band at 1.27–1.30 (g/cm3) and the rest 1/3 at 1.20–1.23 (g/cm3) and below 1.20 (g/cm3). The 1.27–1.30 RNP8 could be separated into two density classes, 1.27–1.26 and 1.30 (g/cm3) respectively. The latter RNP class was apparently complexed with ribosomal components because they were completely converted to the former RNP class [free RNPs) by 25 mM EDTA treatment. Further, the 1.30 (g/cm3) RNPs were res-solved into several RNP species having sedimentation coefficients above 50 S, which were transformed mostly to 20–30 S RNPs in the presence of 25 mM EDTA. The free 20–30 S RNPs contained 8–14 S poly(A)+RNAs, having the highest template activity in a wheat embryo cell-free system, whereas the 1.20–1.23 poly(A)±RNPs consisted of 10 S and 16 S RNPs, both of which contained 4 S poly(A)-containing sequences without any template activity.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.